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Sickle confirm modified hemoglobin solubility test


Tim R. Randolph


The present invention provides a novel test method (Sickle Confirm) to differentiate sickle cell anemia from sickle cell trait for potential use in developing countries. The test comprises: forming a first solution comprising a sample from the subject, a phosphate buffer, a detergent, and a reducing agent; subjecting the first solution to centrifugation to form a second solution and a supernatant; taking a color reading of the supernatant and of the second solution; optionally filtering the second solution to form a filtrate and a precipitate, and optionally measuring the amount of the precipitation and the absorbance of the filtrate or taking a color reading of the filtrate.

Intellectual Property Status

U.S. patent 8,623,659